Cloned (Comment) | Organism |
---|---|
gene BSMT, cloning in Escherichia coli, Arabidopsis thaliana plants are transformed using the floral dip method via Agrobacterium tumefaciens AGL1, recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leading to chlorosis and altered host susceptibility, induced transgenic plants are not able to support large galls and have a brownish appearance of some clubs. The methylester of SA (MeSA) is transported from clubbed Arabidopsis roots to leaves, as shown using heavy isotope-labelled MeSA, and is emitted only from leaves of infected plants, semi- and quantitative RT-PCR expression analysis | Plasmodiophora brassicae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + benzoate | Plasmodiophora brassicae | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | Plasmodiophora brassicae | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodiophora brassicae | R4I7S9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + anthranilate | - |
Plasmodiophora brassicae | methyl anthranilate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + benzoate | - |
Plasmodiophora brassicae | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | - |
Plasmodiophora brassicae | methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzoate-salicylate methyltransferase | - |
Plasmodiophora brassicae |
benzoic acid/salicylic acid methyltransferase | UniProt | Plasmodiophora brassicae |
BSMT | - |
Plasmodiophora brassicae |
More | see also EC 2.1.1.273 | Plasmodiophora brassicae |
PbBSMT methyltransferase | - |
Plasmodiophora brassicae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Plasmodiophora brassicae |
General Information | Comment | Organism |
---|---|---|
malfunction | recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leads to chlorosis and altered host susceptibility. Transcription of PbBSMT is associated with: (1) strong leaf phenotypes from anthocyanin accumulation and chlorosis followed by browning, (2) increased plant susceptibility after infection with Plasmodiophora brassicae that is manifested as more yellow leaves and reduced growth of upper plant parts, and (3) induced transgenic plants are not able to support large galls and had a brownish appearance of some clubs. Microarray data indicate that chlorophyll loss is accompanied by reduced transcription of genes involved in photosynthesis, while genes encoding glucose metabolism, mitochondrial functions and cell wall synthesis are upregulated. Phenotype overview | Plasmodiophora brassicae |
physiological function | the plant pathogenic protist Plasmodiophora brassicae causes clubroot disease of Brassicaceae. The biotrophic organism can downregulate plant defence responses via its salicylic acid methyltransferase. The enzyme is involved in attenuation of host defence responses in the roots by metabolising a plant defence signal. Role for the methylation of salicylic acid in attenuating plant defence response in infected roots as a strategy for intracellular parasitism. Salicylic acid (SA) is a plant defence hormone that acts as a prominent signal in response to biotrophic pathogens | Plasmodiophora brassicae |