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Literature summary for 2.1.1.290 extracted from

  • Suzuki, Y.; Noma, A.; Suzuki, T.; Ishitani, R.; Nureki, O.
    Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4 (2009), Nucleic Acids Res., 37, 2910-2925.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.290

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and enzyme bound to S-adenosyl-L-methionine or S-adenosyl-L-homocysteine, sitting drop vapor diffusion method, using 200 mM ammonium citrate (pH 7.0), 10 mM HEPES (pH 7.5), 20% (w/v) PEG3,350, 20 mM sodium citrate, and 1% (v/v) 2-propanol or microseeding method using 200 mM ammonium citrate (pH 7.0) and 20% (w/v) PEG3350 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
R88A inactive Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72430
-
 2 * 72430, sedimentation velocity analytical ultracentrifugation Saccharomyces cerevisiae
80022
-
 2 * 80022, calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 Saccharomyces cerevisiae
-
 2 S-adenosyl-L-homocysteine + wybutosine
-
 ?
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 Saccharomyces cerevisiae BY4742
-
 2 S-adenosyl-L-homocysteine + wybutosine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08282
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2
-
 Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + wybutosine
-
 ?
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2
-
 Saccharomyces cerevisiae BY4742 2 S-adenosyl-L-homocysteine + wybutosine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 72430, sedimentation velocity analytical ultracentrifugation Saccharomyces cerevisiae
homodimer 2 * 80022, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
tRNA-yW synthesizing enzyme-4
-
 Saccharomyces cerevisiae
TYW4
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on Saccharomyces cerevisiae