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Literature summary for 2.1.1.33 extracted from

  • Hurwitz, J.; Gold, M.; Anders, M.
    The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IV. The properties of the soluble ribonucleic acid-methylating enzymes (1964), J. Biol. Chem., 239, 3474-3482.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates Escherichia coli
additional information not activated by spermine Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by adenosine, L-homocysteine, snake venom phosphodiesterase Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
S-adenosyl-L-homocysteine product inhibition, reversed by S-adenosylmethionine Escherichia coli
Spleen phosphodiesterase
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
S-adenosyl-L-methionine
-
Escherichia coli
0.21
-
methyl deficient tRNA from E. coli
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Escherichia coli
Mg2+ in presence of Mg2+ 2fold increase of activity Escherichia coli
Mn2+ activates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA Escherichia coli additional information
-
?
S-adenosyl-L-methionine + guanine46 in methyl deficient tRNA from E. coli
-
Escherichia coli ?
-
?
S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir
S-adenosyl-L-methionine + guanine46 in tRNA highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir
S-adenosyl-L-methionine + guanine46 in tRNA extend of methylation is directly proportional to the amount of tRNA added, 0.19% of nucleotide residues are methylated Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 dimethylglutarate buffer Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli