Literature summary for 2.1.1.37 extracted from

Vilkaitis, G.; Suetake, I.; Klimasauskas, S.; Tajima, S.
Processive methylation of hemimethylated CpG sites by mouse Dnmt1 DNA methyltransferase (2005), J. Biol. Chem., 280, 64-72.

Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
expression in a baculovirus/Sf9 cell system	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + DNA	Mus musculus	Dnmt1 plays an essential role in the faithful and efficient maintenance of methylation patterns in the mammalian genome	S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P13864	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant forms of full-length Dnmt1 and a truncated form of Dnmt1-(2911620) lacking the binding sites for PCNA and DNA	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + DNA	Dnmt1 plays an essential role in the faithful and efficient maintenance of methylation patterns in the mammalian genome	Mus musculus	S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine	-	?
S-adenosyl-L-methionine + DNA	unmethylated DNA substrates: poly(dIdC)-poly(dIdC), poly(dGdC)-poly(dGdC) or a 520-bp DNA fragment from the p2HhaBsp. Full-length Dnmt1 methylates hemimethylated DNA with high processivity and a fidelity of over 95%, but unmethylated DNA with much less processivity. Dnmt1-(2911620), the truncated form of Dnmt1, shows identical properties to full-length Dnmt1 indicating that the N-terminal 290-amino acid residue region of Dnmt1 is not required for preferential activity toward hemimethylated sites or for processivity of the enzyme. Dnmt1 methylates hemimethylated CpG sites on one strand of double-stranded DNA during a single processive run	Mus musculus	S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine	-	?

Synonyms

Synonyms	Comment	Organism
Dnmt1	-	Mus musculus

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Release 2023.1 (January 2023)