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Literature summary for 2.1.1.37 extracted from
- Residues distal from the active site that alter enzyme function in M.HhaI DNA cytosine methyltransferase (2005), J. Biomol. Struct. Dyn., 22, 533-543.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D73A | 25fold improvement in affinity to S-adenosyl-L-methionine | Haemophilus haemolyticus |
| M168A | 4fold enhancement in the ratio of turnover number/Km-value(DNA, mainly caused be change in Km) | Haemophilus haemolyticus |
| N39A | 4fold enhancement in the ratio of turnover number/Km-value(DNA) | Haemophilus haemolyticus |
| Q301A | 40fold decreased Km-ratio of turnover number/Km-value(DNA) and an 80fold increased Kd(DNA)-value | Haemophilus haemolyticus |
| V282A | 4fold improvement in catalytic turnover | Haemophilus haemolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA | Haemophilus haemolyticus | - |
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus haemolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haemophilus haemolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA | - |
Haemophilus haemolyticus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M.HhaI | - |
Haemophilus haemolyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
S-adenosyl-L-methionine | mutant enzyme R106A, mutant enzyme N173A | Haemophilus haemolyticus | |
| 0.03 | - |
S-adenosyl-L-methionine | mutant enzyme D73A, mutant enzyme M168A, mutant enzyme Q301A | Haemophilus haemolyticus | |
| 0.04 | - |
S-adenosyl-L-methionine | mutant enzyme K111A, mutant enzyme V306A | Haemophilus haemolyticus | |
| 0.05 | - |
S-adenosyl-L-methionine | wild-type enzyme, mutant enzyme D71A | Haemophilus haemolyticus | |
| 0.08 | - |
S-adenosyl-L-methionine | mutant enzyme N39A | Haemophilus haemolyticus | |
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Release 2023.1 (January 2023)
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