General Stability | Organism |
---|---|
-80°C, storage in 30% glycerol stabilizes enzyme | Tetradesmus obliquus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Daucus carota | |
5-fluoro-2'-deoxyuridylic acid | - |
Leishmania major | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
5-fluoro-2'-deoxyuridylic acid | - |
Tetradesmus obliquus | |
Arabinouridylate | - |
Tetradesmus obliquus | |
Hydroxyurea | supresses thymidilate synthase in growing algae, but no effect on purified enzyme | Tetradesmus obliquus | |
methotrexate | - |
Saccharomyces cerevisiae | |
methotrexate | - |
Tetradesmus obliquus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Tetradesmus obliquus |
56000 | - |
2 * 56000 | Leishmania major |
100000 | - |
sedimentation in sucrose density gradients | Tetradesmus obliquus |
110000 | - |
- |
Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Chlamydomonas reinhardtii | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Tetradesmus obliquus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Nicotiana tabacum | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Daucus carota | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Leishmania major | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Abies sp. | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies sp. | - |
pine | - |
Bos taurus | - |
calf | - |
Chlamydomonas reinhardtii | - |
- |
- |
Daucus carota | - |
carrot, wild and culture form | - |
Leishmania major | - |
- |
- |
Mus musculus | - |
- |
- |
Nicotiana tabacum | - |
tobacco | - |
Saccharomyces cerevisiae | - |
bakers' yeast | - |
Tetradesmus obliquus | - |
unicellular green algae | - |
Purification (Comment) | Organism |
---|---|
- |
Tetradesmus obliquus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Mus musculus | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0257 | - |
- |
Tetradesmus obliquus |
Storage Stability | Organism |
---|---|
-80°C, storage in 30% glycerol stabilizes enzyme | Tetradesmus obliquus |
5°C half-life in buffered solution only 2 days | Tetradesmus obliquus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Chlamydomonas reinhardtii | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Chlamydomonas reinhardtii | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tetradesmus obliquus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tetradesmus obliquus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Nicotiana tabacum | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Nicotiana tabacum | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Daucus carota | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Daucus carota | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Abies sp. | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Abies sp. | dihydrofolate + dTMP | - |
r | |
additional information | bifunctional enzyme thymidilate synthase-dihydrofolate reductase, not possible to separate the 2 activities | Tetradesmus obliquus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Saccharomyces cerevisiae |
dimer | - |
Bos taurus |
dimer | 2 * 50000, SDS-PAGE | Tetradesmus obliquus |
dimer | 2 * 56000 | Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Tetradesmus obliquus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Tetradesmus obliquus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity is negligible below pH 6.0 and above pH 9.0 | Tetradesmus obliquus |