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Literature summary for 2.1.1.45 extracted from
- Thymidylate synthetase from Escherichia coli K12. Purification, and dependence of kinetic properties on sugar conformation and size of the 2 substituent (1979), Eur. J. Biochem., 102, 223-230.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
2'-deoxyuridylate | - |
Escherichia coli |  |
| 0.0104 | - |
2'-deoxyuridylate | - |
Escherichia coli | |
| 0.014 | - |
5,10-methylene-5,6,7,8-tetrahydrofolate | - |
Escherichia coli | |
| 0.11 | - |
2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli | |
| 0.113 | - |
2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | - |
Streptococcus pneumoniae | |
| Mg2+ | - |
Lacticaseibacillus casei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000 | Escherichia coli |
| 32500 | - |
2 * 32500, SDS-PAGE | Escherichia coli |
| 63000 | 64000 | gel filtration | Escherichia coli |
| 64000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Streptococcus pneumoniae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli EM 20031 K12 | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
| Escherichia coli EM 20031 K12 | - |
- |
- |
| Escherichia phage T2 | - |
- |
- |
| Lacticaseibacillus casei | - |
- |
- |
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.47 | - |
- |
Escherichia coli |
| 1.52 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme can be stored frozen for several months without significant loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli | 7,8-dihydrofolate + ? | - |
? | |
| 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli B / ATCC 11303 | 7,8-dihydrofolate + ? | - |
? | |
| 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli EM 20031 K12 | 7,8-dihydrofolate + ? | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli EM 20031 K12 | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli EM 20031 K12 | dihydrofolate + dTMP | - |
? | |
| additional information | - |
Streptococcus pneumoniae | ? | - |
? | |
| additional information | - |
Lacticaseibacillus casei | ? | - |
? | |
| additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli | ? | - |
? | |
| additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli | ? | - |
? | |
| additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli EM 20031 K12 | ? | - |
? | |
| additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli EM 20031 K12 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Streptococcus pneumoniae |
| dimer | - |
Lacticaseibacillus casei |
| dimer | - |
Escherichia phage T2 |
| dimer | 2 * 32500, SDS-PAGE | Escherichia coli |
| dimer | 2 * 32000 | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Escherichia coli |
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