Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Tequatrovirus T4 |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Escherichia phage T2 |
Cloned (Comment) | Organism |
---|---|
T4 thymidilate synthase gene in amplification plasmid pKC30 | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Escherichia phage T2 | |
methotrexate | - |
Tequatrovirus T4 | |
Quinazoline | - |
Escherichia phage T2 | |
Quinazoline | - |
Lacticaseibacillus casei | |
Quinazoline | - |
Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Tequatrovirus T4 |
32000 | - |
2 * 32000, SDS-PAGE | Escherichia phage T2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Bacillus subtilis | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Escherichia phage T2 | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Rattus norvegicus | - |
H35 hepatoma cells | - |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified from phage infected E. coli | Tequatrovirus T4 |
purified from phage infected E. coli | Escherichia phage T2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Bacillus subtilis | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bacillus subtilis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bacillus subtilis |
dimer | - |
Escherichia coli |
dimer | - |
Rattus norvegicus |
dimer | - |
Lacticaseibacillus casei |
dimer | 2 * 32000, SDS-PAGE | Tequatrovirus T4 |
dimer | 2 * 32000, SDS-PAGE | Escherichia phage T2 |