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Literature summary for 2.1.1.45 extracted from
- Purification and properties of T4 phage thymidylate synthetase produced by the cloned gene in an amplification vector (1983), J. Biol. Chem., 258, 2045-2051.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
| medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Tequatrovirus T4 |
| medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Escherichia phage T2 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| T4 thymidilate synthase gene in amplification plasmid pKC30 | Tequatrovirus T4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Escherichia phage T2 |  |
| methotrexate | - |
Tequatrovirus T4 | |
| Quinazoline | - |
Escherichia phage T2 | |
| Quinazoline | - |
Lacticaseibacillus casei | |
| Quinazoline | - |
Tequatrovirus T4 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Tequatrovirus T4 |
| 32000 | - |
2 * 32000, SDS-PAGE | Escherichia phage T2 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Bacillus subtilis | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
| Escherichia phage T2 | - |
- |
- |
| Lacticaseibacillus casei | - |
- |
- |
| Rattus norvegicus | - |
H35 hepatoma cells | - |
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from phage infected E. coli | Tequatrovirus T4 |
| purified from phage infected E. coli | Escherichia phage T2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bacillus subtilis | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bacillus subtilis | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Bacillus subtilis |
| dimer | - |
Escherichia coli |
| dimer | - |
Rattus norvegicus |
| dimer | - |
Lacticaseibacillus casei |
| dimer | 2 * 32000, SDS-PAGE | Tequatrovirus T4 |
| dimer | 2 * 32000, SDS-PAGE | Escherichia phage T2 |
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