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Literature summary for 2.1.1.45 extracted from
- Characterization of thymidylate synthetase and dihydrofolate reductase from Plasmodium berghei (1984), Int. J. Parasitol., 14, 513-520.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Homo sapiens |
| medicine | - |
Crithidia fasciculata |
| medicine | target enzyme for antitumor and antibacterial agents | Plasmodium berghei |
| medicine | target enzyme for malarial chemotherapy | Plasmodium berghei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-2'-deoxyuridylic acid | - |
Plasmodium berghei |  |
| KCl | - |
Plasmodium berghei | |
| methotrexate | - |
Homo sapiens | |
| methotrexate | - |
Plasmodium berghei | |
| NaCl | - |
Plasmodium berghei | |
| Urea | - |
Plasmodium berghei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0714 | - |
2'-deoxyuridylate | - |
Plasmodium berghei | |
| 0.3125 | - |
5,10-methylenetetrahydrofolate | - |
Plasmodium berghei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Plasmodium berghei |
| 132000 | - |
gel filtration | Plasmodium berghei |
| 150000 | - |
gel filtration | Crithidia fasciculata |
| 150000 | - |
gel filtration | Plasmodium berghei |
| 150000 | - |
gel filtration | Leishmania major |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Streptococcus pneumoniae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Leishmania major | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Plasmodium berghei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human | - |
| Lacticaseibacillus casei | - |
- |
- |
| Leishmania major | - |
- |
- |
| Plasmodium berghei | - |
- |
- |
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| partially, copurified with dihydrofolate reductase | Plasmodium berghei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blast cell | from patients with acute myelocytic leukemia | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00235 | - |
- |
Plasmodium berghei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme loses half of its activity upon storage for a few days | Plasmodium berghei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium berghei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Plasmodium berghei | dihydrofolate + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | - |
Homo sapiens |
| dimer | - |
Streptococcus pneumoniae |
| dimer | - |
Lacticaseibacillus casei |
| dimer | - |
Leishmania major |
| dimer | 2 * 65000, SDS-PAGE | Plasmodium berghei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | - |
Plasmodium berghei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | stable in this range but loses activity completely below pH 3.5 or above pH 11.5 | Plasmodium berghei |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Plasmodium berghei | |
| 0.103 | - |
methotrexate | uncompetitive with dUMP | Plasmodium berghei | |
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