Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | - |
Mus musculus | |
beta-mercaptoethanol | essential for maximum activity | Kitasatospora aureofaciens | |
dithioerythritol | - |
Kitasatospora aureofaciens | |
dithiothreitol | - |
Mus musculus | |
dithiothreitol | - |
Escherichia coli | |
dithiothreitol | - |
Kitasatospora aureofaciens |
General Stability | Organism |
---|---|
glycerol and Triton X-100 stabilize the enzyme | Kitasatospora aureofaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridylic acid | - |
Kitasatospora aureofaciens | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
2'-deoxyuridylate | - |
Crithidia fasciculata | |
0.00378 | - |
2'-deoxyuridylate | - |
Kitasatospora aureofaciens | |
0.0211 | - |
N5,N10-methylenetetrahydrofolate | - |
Kitasatospora aureofaciens | |
0.0253 | - |
methylenetetrahydrofolate | - |
Mus musculus | |
0.0389 | - |
NADPH | - |
Kitasatospora aureofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | stimulates activity | Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Kitasatospora aureofaciens |
77000 | - |
gel filtration | Kitasatospora aureofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Kitasatospora aureofaciens | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Crithidia fasciculata | - |
- |
- |
Escherichia coli | - |
- |
- |
Kitasatospora aureofaciens | - |
industrial strain BMK | - |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Saccharomyces cerevisiae | - |
bakers' yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00652 | - |
- |
Kitasatospora aureofaciens |
Storage Stability | Organism |
---|---|
-20°C, TS activity of crude enzyme remains unchanged for 1 month of storage, purified enzyme loses 50% of its activity within 3-4 weeks | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Kitasatospora aureofaciens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Kitasatospora aureofaciens | dihydrofolate + dTMP | - |
r | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | monofunctional enzyme | Kitasatospora aureofaciens | ? | - |
? | |
[6R,S]-5,10-methylenetetrahydropteroylglutamate + 2'-deoxyuridylate | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Escherichia coli |
dimer | - |
Saccharomyces cerevisiae |
dimer | - |
Bos taurus |
dimer | 2 * 28000, SDS-PAGE | Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Crithidia fasciculata |
30 | - |
- |
Kitasatospora aureofaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | - |
Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mus musculus |
7 | - |
- |
Escherichia coli |
7 | - |
- |
Saccharomyces cerevisiae |
7 | - |
- |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | absolute requirement | Kitasatospora aureofaciens |