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Literature summary for 2.1.1.45 extracted from

  • Carlson, J.H.; Hill, S.A.
    Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae (1997), FEMS Microbiol. Lett., 151, 225-230.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
thyA gene cloned, sequenced and expressed in Escherichia coli KLC1597, transformation with pBr322 Neisseria gonorrhoeae

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridylic acid
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Haemophilus influenzae
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Bacillus subtilis
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Neisseria gonorrhoeae biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Neisseria gonorrhoeae MS11 biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Haemophilus influenzae
-
-
-
Neisseria gonorrhoeae
-
-
-
Neisseria gonorrhoeae MS11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Haemophilus influenzae dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Haemophilus influenzae dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Bacillus subtilis dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Bacillus subtilis dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Neisseria gonorrhoeae dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Neisseria gonorrhoeae dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Neisseria gonorrhoeae MS11 dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Neisseria gonorrhoeae MS11 dihydrofolate + dTMP
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
5-fluoro-2'-deoxyuridylic acid
-
Bacillus subtilis