Cloned (Comment) | Organism |
---|---|
plasmid containing the mutation was transformed into the Escherichia coli TX61-(thyA-) bacterial strain that does not produce its own thymidylate synthase enzyme | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
grown in low-salt conditions, 100 mM Tris, pH 9.0, 20 mM beta-mercaptoethanol, 3 mM KH2PO4, and 10-20% PEG 4K, by hanging drop diffusion at 4 °C | Homo sapiens |
wild-type and mutant R163K. Data show that all subunits of the mutant are in the active conformation, while wild-type crystallizes as the inactive conformer. Structure reveals differences in the environment of catalytic residue Cys195 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R163K | mutation results in destabilization of inactive conformation. Activity is about 33% higher than in wild-type. Crystallization data show that all subunits of the mutant are in the active conformation, while wild-type crystallizes as the inactive conformer. Structure reveals differences in the environment of catalytic residue Cys195 | Homo sapiens |
R163K | the activity of this mutant is 33% higher than that of wt hTS | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
7,8-dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04818 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | 7,8-dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | mTHF, TS is the sole source of de novo thymidylate which is required for DNA synthesis | Homo sapiens | 7,8-dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | asymmetric, X-ray diffraction | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HTS | - |
Homo sapiens |