Literature summary for 2.1.1.45 extracted from

Codacci-Pisanelli, G.; Noordhuis, P.; van der Wilt, C.L.; Peters, G.J.
Selective protection by uridine of growth inhibition by 5-fluorouracil (5FU) mediated by 5FU incorporation into RNA, but not the thymidylate synthase mediated growth inhibition by 5FU-leucovorin (2008), Nucleosides Nucleotides Nucleic Acids, 27, 733-739.

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Inhibitors

Inhibitors	Comment	Organism	Structure
5-fluorouracil	WiDr cells: 0.005 mM inhibits growth to 22%, inhibition cannot be potentiated by leucovorin but is rescued by uridine, 0.025 mM inhibits growth to 10%, inhibition can be rescued badly by uridine to an value of 31%, SW-948 and HT-29 cells: 0.005 mM shows an antiproliferative activity that is not affected by leucovorine addition and can be rescued by uridine, 0.1 mM shows a complete inhibition that cannot be rescued by uridine	Homo sapiens
5-fluorouracil	0.001 mM inhibits growth to 13%, inhibition can be rescued by uridine, 0.005 mM inhibits growth completely, inhibition cannot be rescued by uridine	Mus musculus
additional information	5FU-LV, combination of 5-fluorouracil and leucovorin	Homo sapiens
additional information	combination of 5-FU and leucovorin, enhances the inhibition of thymidylate synthase, 0.001 mM inhibits growth to only 1%, inhibition can be rescued by uridine to an almost normal value of 83%	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	Mus musculus	-	7,8-dihydrofolate + dTMP	-	?
5,10-methylenetetrahydrofolate + dUMP	Homo sapiens	-	7,8-dihydrofolate + dTMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
C26-10 cell	colon cancer cell	Mus musculus	-
Colon-26 cell	-	Mus musculus	-
HT-29 cell	-	Homo sapiens	-
SW-948 cell	-	Homo sapiens	-
WiDr cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	-	Mus musculus	7,8-dihydrofolate + dTMP	-	?
5,10-methylenetetrahydrofolate + dUMP	-	Homo sapiens	7,8-dihydrofolate + dTMP	-	?

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Release 2023.1 (January 2023)