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Literature summary for 2.1.1.45 extracted from
- Crystallization and preliminary crystallographic studies of dihydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen (2009), Acta Crystallogr. Sect. F, 65, 1175-1178.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil, and Q-8, microbatch method, using 0.1-0.2 M ammonium acetate, 20-22% (w/v) PEG 4000 and 0.1 M sodium citrate buffer pH 5.6 | Trypanosoma cruzi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58800 | - |
calculated from amino acid sequence | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| methotrexate affinity column chromatography | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHFR-TS | - |
Trypanosoma cruzi |
| dihydrofolate reductase-thymidylate synthase | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| N5,N10-methylenetetrahydrofolate | - |
Trypanosoma cruzi | |
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