Literature summary for 2.1.1.45 extracted from

Bielas, J.H.; Schmitt, M.W.; Icreverzi, A.; Ericson, N.G.; Loeb, L.A.
Molecularly evolved thymidylate synthase inhibits 5-fluorodeoxyuridine toxicity in human hematopoietic cells (2009), Hum. Gene Ther., 20, 1703-1707.

Search for more literature for 2.1.1.45

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain K562	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A17T/D116A/D254E	the mutant shows decreased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
D254N	the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
G52S	the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
T51S	the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
T51S/G52S	the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
T51S/K82Q/K99E/N171S	the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens
T53S/Y258F	the mutant shows sensitivity towards 5-fluorodeoxyuridine similar to the wild type enzyme	Homo sapiens
Y258F	the mutant shows decreased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
5-fluorodeoxyuridine	forms an inhibitory, ternary covalent complex with the enzyme	Homo sapiens
5-fluorouracil	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hematopoietic cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	-	Homo sapiens	dihydrofolate + dTMP	-	?

Cofactor

Cofactor	Comment	Organism	Structure
N5,N10-methylenetetrahydrofolate	-	Homo sapiens

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Release 2023.1 (January 2023)