Literature summary for 2.1.1.45 extracted from

Chanama, M.; Chanama, S.; Shaw, P.J.; Chitnumsub, P.; Leartsakulpanich, U.; Yuthavong, Y.
Formation of catalytically active cross-species heterodimers of thymidylate synthase from Plasmodium falciparum and Plasmodium vivax (2011), Mol. Biol. Rep., 38, 1029-1037.

Search for more literature for 2.1.1.45

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells	Plasmodium falciparum
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells	Plasmodium vivax

Protein Variants

Protein Variants	Comment	Organism
C490A	inactive	Plasmodium falciparum
C506A	inactive	Plasmodium vivax
C506D	inactive	Plasmodium vivax
C506F	inactive	Plasmodium vivax
C506P	inactive	Plasmodium vivax
C506Y	inactive	Plasmodium vivax
R470D	inactive	Plasmodium falciparum
R486D	inactive	Plasmodium vivax
R486L	inactive	Plasmodium vivax
R486Y	inactive	Plasmodium vivax

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-
Plasmodium vivax	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
methotrexate-Sepharose column chromatography and anion exchange column chromatography	Plasmodium falciparum
methotrexate-Sepharose column chromatography and anion exchange column chromatography	Plasmodium vivax

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	-	Plasmodium falciparum	dihydrofolate + dTMP	-	?
5,10-methylenetetrahydrofolate + dUMP	-	Plasmodium vivax	dihydrofolate + dTMP	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Plasmodium falciparum
homodimer	-	Plasmodium vivax

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)