Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography, Toyopearl-SuperQ column chromatography, ammonium sulfate precipitation, and Toyopearl-ether 650M column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity | Thermus thermophilus | ? | - |
? | |
additional information | TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase | Thermus thermophilus | ? | - |
? | |
additional information | TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
additional information | TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O6-methylguanine-DNA methyltransferase | - |
Thermus thermophilus |
OGT | - |
Thermus thermophilus |
TTHA1564 | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme requires no cofactor | Thermus thermophilus |