Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 Blue cells | Escherichia coli |
expressed in Escherichia coli XL-1 Blue cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construct human AGT-03 (where AGT sequence -V149CSSGAVGN157- is replaced with the corresponding Ogt -I143GRNGTMTG151-), exhibits enhanced DNA containing 4-O-methylthymine repair activity in vitro compared to wild type AGT | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | P16455 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity column chromatography | Escherichia coli |
immobilized metal affinity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 4-O-methylthymine + [protein]-L-cysteine | - |
Escherichia coli | DNA lacking 4-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Escherichia coli | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
additional information | human AGT acts poorly on DNA containing 4-O-methylthymine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Escherichia coli |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |
OGT | - |
Escherichia coli |