Literature summary for 2.1.1.72 extracted from

Kossykh, V.G.; Schlagman, S.L.; Hattman, S.
Phage T4 DNA [N6-adenine]methyltransferase. Overexpression, purification, and characterization (1995), J. Biol. Chem., 270, 14389-14393.

Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment)	Organism
the enzyme is subcloned into the plasmid expression vector pJW2	Tequatrovirus T4

Inhibitors

Inhibitors	Comment	Organism	Structure
methylated DNA	competitive	Tequatrovirus T4
additional information	inhibited at ionic strength greater than 0.2 M	Tequatrovirus T4
NEM	-	Tequatrovirus T4
S-adenosyl-L-homocysteine	competitive	Tequatrovirus T4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0000000011	-	adenine in T4 gt- dam- DNA	nonglucosylated unmethylated T4 gt- dam- DNA	Tequatrovirus T4
0.0001	-	S-adenosyl-L-methionine	-	Tequatrovirus T4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	no requirement for Mg2+	Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30400	-	x * 30400, SDS-PAGE	Tequatrovirus T4
30690	-	gel filtration, glycerol gradient centrifugation	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Tequatrovirus T4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA	-	Tequatrovirus T4	S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA	-	?
S-adenosyl-L-methionine + DNA adenine	nonglucosylated unmethylated T4 gt- dam- DNA. The enzyme methylates the palindromic tetranucleotide, GATC, designated the canonical sequence. At high Mtase:DNA ratios, T4 Dam can methylate some noncanonical sequences belonging to GAY, where Y represents cytosine or thymine	Tequatrovirus T4	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?

Subunits

Subunits	Comment	Organism
?	x * 30400, SDS-PAGE	Tequatrovirus T4

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Tequatrovirus T4

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000000046	-	methylated DNA	-	Tequatrovirus T4
0.0024	-	S-adenosyl-L-homocysteine	-	Tequatrovirus T4

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Release 2023.1 (January 2023)