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Literature summary for 2.1.1.79 extracted from

  • Iwig, D.F.; Grippe, A.T.; McIntyre, T.A.; Booker, S.J.
    Isotope and elemental effects indicate a rate-limiting methyl transfer as the initial step in the reaction catalyzed by Escherichia coli cyclopropane fatty acid synthase (2004), Biochemistry, 43, 13510-13524.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0564
-
Se-adenosyl-L-selenomethionine
-
Escherichia coli
0.0894
-
S-adenosyl-L-methionine
-
Escherichia coli
0.74
-
Te-adenosyl-L-telluromethionine
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
-78°C, 25% glycerol, less than 10% loss of activity after 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] reaction takes place via methyl transfer followed by proton loss, rather than by processes that are initiated by proton abstraction from S-adenosyl-L-methionine. Methyl transfer takes place via a tight SN2 transition state Escherichia coli S-adenosyl-L-homocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
?
Se-adenosyl-L-selenomethionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
Escherichia coli Se-adenosyl-L-selenohomocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
?
Te-adenosyl-L-telluromethionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
Escherichia coli Te-adenosyl-L-tellurohomocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
?

Synonyms

Synonyms Comment Organism
CFA synthase
-
Escherichia coli
cyclopropane fatty acid synthase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
Te-adenosyl-L-telluromethionine
-
Escherichia coli
0.12
-
S-adenosyl-L-methionine
-
Escherichia coli
0.217
-
Se-adenosyl-L-selenomethionine
-
Escherichia coli