Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3), together with chaperone-coding genes (plasmid pG-KJE8) | Phaeodactylum tricornutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
thiocyanate | pH 7.0, 30°C | Phaeodactylum tricornutum | |
9.9 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration, His-tagged protein | Phaeodactylum tricornutum |
34000 | - |
1 * 34000, SDS-PAGE, His-tagged protein | Phaeodactylum tricornutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeodactylum tricornutum | B7FWR0 | - |
- |
Phaeodactylum tricornutum CCAP 1055/1 | B7FWR0 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Phaeodactylum tricornutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1043 | - |
substrate iodometane, pH 7.0, 30°C | Phaeodactylum tricornutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + thiocyanate | - |
Phaeodactylum tricornutum | S-adenosyl-L-homocysteine + methyl thiocyanate | - |
? | |
S-adenosyl-L-methionine + thiocyanate | - |
Phaeodactylum tricornutum CCAP 1055/1 | S-adenosyl-L-homocysteine + methyl thiocyanate | - |
? | |
SH- + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum | CH3SH + S-adenosyl-L-homocysteine | - |
? | |
SH- + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum CCAP 1055/1 | CH3SH + S-adenosyl-L-homocysteine | - |
? | |
thiocyanate + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum | methyl thiocyanate + S-adenosyl-L-homocysteine | - |
? | |
thiocyanate + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum CCAP 1055/1 | methyl thiocyanate + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE, His-tagged protein | Phaeodactylum tricornutum |
Synonyms | Comment | Organism |
---|---|---|
S-adenosyl-L-methionine-dependent halide/thiol methyltransferase | - |
Phaeodactylum tricornutum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
thiocyanate | pH 7.0, 30°C | Phaeodactylum tricornutum | |
0.0381 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
50% activity at pH 6.0 or pH 8.5 | Phaeodactylum tricornutum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00129 | - |
SCN- | pH 7.0, 30°C | Phaeodactylum tricornutum | |
0.00385 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum |