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Literature summary for 2.1.2.1 extracted from

  • Mitchell, M.K.; Reynolds, P.H.S.; Blevins, D.G.
    Serine hydroxymethyltransferase from soybean root nodules (1986), Plant Physiol., 81, 553-557.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
L-serine stabilizes Glycine max

Inhibitors

Inhibitors Comment Organism Structure
5,10-methenyltetrahydrofolate
-
Glycine max
5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
Glycine max
5-methyl-5,6,7,8-tetrahydrofolate
-
Glycine max
glycine
-
Glycine max
KCl
-
Glycine max
additional information not: methionine, S-adenosyl-L-methionine, XMP, IMP, phosphoglycerate Glycine max
NaCl
-
Glycine max
tetrahydrofolate derivatives
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Glycine max
0.25
-
tetrahydrofolate
-
Glycine max
1.5
-
L-serine
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Glycine max
230000
-
-
Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Glycine max main glycine source for purine biosynthetic pathway in ureide biogenesis glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate Glycine max L. Merr. cv. Williams main glycine source for purine biosynthetic pathway in ureide biogenesis glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-
Glycine max L. Merr. cv. Williams
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
nodule inoculated with Rhizobium japonicum strain 311b142 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Glycine max
11.9
-
-
Glycine max

Storage Stability

Storage Stability Organism
-20°C, 8 weeks Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Glycine max glycine + 5,10-methylenetetrahydrofolate + H2O
-
r
L-serine + tetrahydrofolate main glycine source for purine biosynthetic pathway in ureide biogenesis Glycine max glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate
-
Glycine max L. Merr. cv. Williams glycine + 5,10-methylenetetrahydrofolate + H2O
-
r
L-serine + tetrahydrofolate main glycine source for purine biosynthetic pathway in ureide biogenesis Glycine max L. Merr. cv. Williams glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 55000, SDS-PAGE Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 about half-maximal activity at pH 7.3 and about 60% of maximal activity at 9.5 Glycine max
9.5
-
no activity above Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8
-
5-methyl-5,6,7,8-tetrahydrofolate
-
Glycine max
2.9
-
5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
Glycine max
3
-
glycine
-
Glycine max
3
-
5,10-methenyltetrahydrofolate
-
Glycine max