Literature summary for 2.1.2.1 extracted from

Bhavani, B.S.; Rajaram, V.; Bisht, S.; Kaul, P.; Prakash, V.; Murthy, M.R.; Appaji Rao, N.; Savithri, H.S.
Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage (2008), FEBS J., 275, 4606-4619.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of Y51F and Y61A SHMT mutants are obtained by hanging drop vapour diffusion using 50% (v/v) 2-methyl 2,4-pentanediol as the precipitant	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
Y51F	the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate-independent activities	Geobacillus stearothermophilus
Y61A	the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate independent activities	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and DEAE-cellulose column chromatography	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.05	-	mutant enzyme Y61A, using L-serine as substrate, at 37°C	Geobacillus stearothermophilus
5	-	wild type enzyme, using L-serine as substrate, at 37°C	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + tetrahydrofolate	-	Geobacillus stearothermophilus	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
serine hydroxymethyltransferase	-	Geobacillus stearothermophilus
SHMT	-	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	wild type enzyme contains 1 mol per mole of subunit	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)