Literature summary for 2.1.2.1 extracted from

Florio, R.; Chiaraluce, R.; Consalvi, V.; Paiardini, A.; Catacchio, B.; Bossa, F.; Contestabile, R.
Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase - the role of evolutionarily conserved hydrophobic contacts (2009), FEBS J., 276, 7319-7328.

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Protein Variants

Protein Variants	Comment	Organism
L276A	the mutation has the effect of lowering the cooperativity of urea denaturation process	Escherichia coli
L785A/L276A	the mutation has the effect of lowering the cooperativity of urea denaturation process	Escherichia coli
L85A	the mutation affect the quaternary structure stability of SHMT	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Urea	in 1 M urea, almost all the cofactor is bound to the enzyme as internal aldimine, indicating that the loss of activity does not result from the denaturation of the active site, these observations suggest that urea might act as an enzyme inhibitor	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
91000	-	SDS-PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A825	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography	Escherichia coli

Renatured (Commentary)

Renatured (Comment)	Organism
denatured SHMT samples (0.023 mM in 8 M urea at 20°C) are able to recover after 4 h when kept with 8 M urea in 50 mM Na-HEPES, pH 7.2, containing 0.2 mM dithiothreitol and 0.1 mM EDTA for 15 h at 20°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + tetrahydrofolate	-	Escherichia coli	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
serine hydroxymethyltransferase	-	Escherichia coli
SHMT	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Escherichia coli

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Release 2023.1 (January 2023)