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Literature summary for 2.1.2.1 extracted from

  • Tramonti, A.; Nardella, C.; di Salvo, M.L.; Barile, A.; Cutruzzola, F.; Contestabile, R.
    Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison full kinetic characterization and substrate inhibition properties (2018), Biochemistry, 57, 6984-6996 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-serine
-
Homo sapiens
tetrahydrofolate isoform SHMT2 maintains a pronounced tetrahydrofolate substrate inhibition even at the alkaline pH characteristic of the mitochondrial matrix Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
0.033
-
tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
0.223
-
tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
0.291
-
5,10-methylenetetrahydrofolate isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
0.45
-
L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
0.556
-
glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
0.66
-
glycine isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
0.98
-
5,10-methylenetetrahydrofolate isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
2.2
-
L-serine isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
4.8
-
L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform SHMT1 Homo sapiens 5829
-
mitochondrion isoform SHMT2 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine Homo sapiens
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens P34896
-
-
Homo sapiens P34897
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Homo sapiens tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine
-
Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Homo sapiens
SHMT1 isoform Homo sapiens
SHMT2 isoform Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.188
-
glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
6.23
-
tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
13.55
-
tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
14.17
-
tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens
8.5
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.258
-
tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
0.368
-
L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
0.602
-
L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.33
-
glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
0.52
-
5,10-methylenetetrahydrofolate isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
0.65
-
5,10-methylenetetrahydrofolate isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
0.77
-
glycine isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
1.33
-
L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
6.5
-
L-serine isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
30.17
-
L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
63.5
-
tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
188.83
-
tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
423.33
-
tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens