Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant H135N/R137A/E168N, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 1.0 M sodium citrate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H135N/R137A | dimeric catalytically active mutant | Homo sapiens |
H135N/R137A/E168N | tetrameric active mutant with a modified, less stable, tetrameric interface | Homo sapiens |
K257Q/Y82A/Y83F | tetrameric inactive mutant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-serine | wild type enzyme SHMT1, at pH 7.2 and 20°C | Homo sapiens | |
0.314 | - |
L-serine | mutant H135N/R137A/E168N, at pH 7.2 and 20°C | Homo sapiens | |
0.57 | - |
L-serine | mutant H135N/R137A, at pH 7.2 and 20°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34896 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
lung cancer cell | - |
Homo sapiens | - |
NCI-H1299 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
SHMT1 | - |
Homo sapiens |
SHMT2alpha | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme dynamically changes the fluxes of one-carbon metabolism by reversibly converting L-serine and tetrahydrofolate into 5,10-methylene-tetrahydrofolate and glycine. The cytosolic isoforms can also translocate to the nucleus to sustain de novo thymidylate synthesis and support cell proliferation | Homo sapiens |