Literature summary for 2.1.2.1 extracted from

Scaletti, E.; Jemth, A.S.; Helleday, T.; Stenmark, P.
Structural basis of inhibition of the human serine hydroxymethyltransferase SHMT2 by antifolate drugs (2019), FEBS Lett., 593, 1863-1873 .

Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)-TIR cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 28% (w/v) PEGMME 2000 (in complex with lometrexol and methotrexate), 0.2 M LiCl2, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with pemetrexed), and 0.2 M NaCl, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with raltitrexed)	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
lometrexol	49.7% residual activity at 0.1 mM	Homo sapiens
methotrexate	87.4% residual activity at 0.1 mM	Homo sapiens
pemetrexed	79.2% residual activity at 0.1 mM	Homo sapiens
raltitrexed	94% residual activity at 0.1 mM	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + glycine + H2O	Homo sapiens	-	tetrahydrofolate + L-serine	-	r
tetrahydrofolate + L-serine	Homo sapiens	-	5,10-methylenetetrahydrofolate + glycine + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P34897	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography and Superdex 75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + glycine + H2O	-	Homo sapiens	tetrahydrofolate + L-serine	-	r
tetrahydrofolate + L-serine	-	Homo sapiens	5,10-methylenetetrahydrofolate + glycine + H2O	-	r

Subunits

Subunits	Comment	Organism
tetramer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
serine hydroxymethyltransferase	-	Homo sapiens
SHMT2	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Homo sapiens

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Release 2023.1 (January 2023)