Cloned (Comment) | Organism |
---|---|
harboring expression vector pJS167, encoding the purN gene | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(6S)-N10-formyltetrahydrofolate | - |
Escherichia coli | |
beta-thioglycinamide dideazafolate | - |
Escherichia coli | |
dideazafolate | - |
Escherichia coli | |
N10-(Bromoacetyl)-5,8-dideazafolate | - |
Escherichia coli | |
N10-formyl-2,4-diaminodideazafolate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
beta-glycinamide ribonucleotide | pH 8.5, tetrahydrofolate as cofactor | Escherichia coli | |
0.0122 | - |
beta-glycinamide ribonucleotide | pH 7.5, tetrahydrofolate as cofactor | Escherichia coli | |
0.0167 | - |
N10-formyldideazafolate | pH 7.5 | Escherichia coli | |
0.0192 | - |
beta-glycinamide ribonucleotide | pH 7.5, dideazafolate as cofactor | Escherichia coli | |
0.0235 | - |
beta-glycinamide ribonucleotide | pH 8.5, dideazafolate as cofactor | Escherichia coli | |
0.0365 | - |
N10-formyldideazafolate | pH 8.5 | Escherichia coli | |
0.0465 | - |
carbocyclic beta-glycinamide ribonucleotide | - |
Escherichia coli | |
0.0775 | - |
(6R)-N10-formyltetrahydrofolate | pH 8.5 | Escherichia coli | |
0.0848 | - |
(6R)-N10-formyltetrahydrofolate | pH 7.5 | Escherichia coli | |
0.15 | - |
N10-Formyl-8-deazafolate | - |
Escherichia coli | |
0.19 | - |
N10-formyl-5-deazafolate | - |
Escherichia coli | |
0.6 | - |
N10-formylfolate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21060 | - |
calculated from peptide sequence deduced from the cDNA | Bacillus subtilis |
23240 | - |
calculated from peptide sequence deduced from the cDNA | Escherichia coli |
24000 | - |
equilibrium sedimentation ultracentrifugation | Escherichia coli |
145000 | - |
- |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Cricetulus griseus | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Bacillus subtilis | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Saccharomyces cerevisiae | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Drosophila melanogaster | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Mus musculus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Escherichia coli | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Homo sapiens | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Cricetulus griseus | - |
chinese hamster | - |
Drosophila melanogaster | - |
fruit fly | - |
Escherichia coli | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | - |
human | - |
Mus musculus | - |
mouse | - |
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | O cell | Homo sapiens | - |
L-1210 cell | murine leukemia | Mus musculus | - |
L-5178-Y cell | murine lymphoma cell line | Mus musculus | - |
ovary | - |
Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.6 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | (6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | (6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Mus musculus | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Escherichia coli | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Homo sapiens | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Cricetulus griseus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Drosophila melanogaster | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Bacillus subtilis | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Saccharomyces cerevisiae | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Drosophila melanogaster | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide | - |
Escherichia coli | tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide | - |
? | |
additional information | - |
Bacillus subtilis | ? | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase | Gallus gallus | ? | - |
? | |
additional information | 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase | Mus musculus | ? | - |
? | |
additional information | 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase | Homo sapiens | ? | - |
? | |
N10-formyl-5-deazafolate + glycinamide ribonucleotide | - |
Escherichia coli | formylglycinamide ribonucleotide + 5-deazafolate | - |
? | |
N10-formyl-8-deazafolate + glycinamide ribonucleotide | - |
Escherichia coli | formylglycinamide ribonucleotide + 8-deazafolate | - |
? | |
N10-formyldideazafolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | dideazafolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
N10-formylfolate + glycinamide ribonucleotide | - |
Escherichia coli | folate + formylglycinamide ribonucleotide | - |
? | |
N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O | - |
Escherichia coli | N10-formyltetrahydrofolate + formylglycinamide ribotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Gallus gallus |
monomer | - |
Bacillus subtilis |
monomer | - |
Mus musculus |
monomer | - |
Homo sapiens |
monomer | - |
Saccharomyces cerevisiae |
monomer | 1 + 25000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
N10-formylfolate | - |
Escherichia coli | |
0.069 | - |
N10-Formyl-8-deazafolate | - |
Escherichia coli | |
1.52 | - |
N10-formyl-5-deazafolate | - |
Escherichia coli | |
13.5 | - |
(6R)-N10-formyltetrahydrofolate | pH 7.5 | Escherichia coli | |
16.1 | - |
N10-formyl-5,8-dideazafolate | pH 7.5 | Escherichia coli | |
20.7 | - |
(6R)-N10-formyltetrahydrofolate | pH 8.5 | Escherichia coli | |
33 | - |
carbocyclic glycinamide ribonucleotide | - |
Escherichia coli | |
40 | - |
N10-formyl-5,8-dideazafolate | pH 8.5 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
L-(-)-10-formyltetrahydrofolate | - |
Gallus gallus | |
L-(-)-10-formyltetrahydrofolate | - |
Escherichia coli |