Cloned (Comment) | Organism |
---|---|
cDNA encoding formiminotransferase cyclodeaminase is cloned and sequenced | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmic surface of the Golgi apparatus in vivo, cytoplasmically oriented peripheral membrane protein of the Golgi membranes | Rattus norvegicus | 139 | - |
microtubule | formiminotransferase cyclodeaminase binds bovine brain microtubules, but not rat liver microtubules in vitro | Rattus norvegicus | 5874 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
480000 | - |
approximately, native octameric bifunctional enzyme with formiminotransferase and cyclodeaminase activity, EC 2.1.2.5 and EC 4.3.1.4 | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formiminoglutamate + tetrahydrofolate | Rattus norvegicus | bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
N-formiminoglutamate + tetrahydrofolate | Rattus norvegicus | histidine metabolism pathway, reaction occurs in vivo within the soluble phase of cytoplasm | 5-formiminotetrahydrofolate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | richest tissue source of formiminotransferase cyclodeaminase, liver-specific enzyme | Rattus norvegicus | - |
additional information | undetectable in adrenal gland, blood cells, marrow cells, brain, epididymis, heart, small intestine, kidney, lung, skeletal muscle, spleen and testis | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 58K: formiminotransferase cyclodeaminase, bifunctional enzyme catalyzing two consecutive steps in the modification of tetrahydrofolate to 5,10-methenyl tetrahydrofolate | Rattus norvegicus | ? | - |
? | |
N-formiminoglutamate + tetrahydrofolate | bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo | Rattus norvegicus | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
N-formiminoglutamate + tetrahydrofolate | histidine metabolism pathway, reaction occurs in vivo within the soluble phase of cytoplasm | Rattus norvegicus | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
tetrahydrofolate + N-formimino-L-glutamate | transfer of a single carbon from formiminoglutamate to tetrahydrofolate, product is 5-formiminotetrahydrofolate | Rattus norvegicus | 5-formiminotetrahydrofolate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | bifunctional formiminotransferase cyclodeaminase | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | - |
Rattus norvegicus |