Literature summary for 2.1.2.8 extracted from

Graves, K.L.; Hardy, L.W.
Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4 (1994), Biochemistry, 33, 13049-13056.

Search for more literature for 2.1.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
structural gene 42 encodes enzyme	Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
D179A	mutant not inactivated by 5-fluoro-dUMP	Tequatrovirus T4
D179N	mutant with altered substrate preference, favoring dUMP rather than dCMP	Tequatrovirus T4
D179N	mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP	Tequatrovirus T4
D179S	mutant not inactivated by 5-fluoro-dUMP	Tequatrovirus T4

Inhibitors

Inhibitors	Comment	Organism	Structure
5-fluorodeoxyuridylate	inactivates by formation of a covalent complex containing enzyme, inhibitor and methylenetetrahydrofolate in a time-dependent manner, 1 mol 5-fluoro-dUMP per enzyme monomer, no substantial inhibition of wild-type enzyme in vivo, inhibition mechanism	Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28600	-	x * 28600	Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	Tequatrovirus T4	-	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4	?

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	Escherichia coli JM101 infected with bacteriophage T4	-

Reaction

Reaction	Comment	Organism	Reaction ID
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	mechanism	Tequatrovirus T4	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	-	Tequatrovirus T4	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4	?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	Cys-148 is catalytically essential	Tequatrovirus T4	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	-	r

Subunits

Subunits	Comment	Organism
?	x * 28600	Tequatrovirus T4

Cofactor

Cofactor	Comment	Organism	Structure
5,10-methylenetetrahydrofolate	-	Tequatrovirus T4
tetrahydrofolate	-	Tequatrovirus T4

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Release 2023.1 (January 2023)