KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-methionyl-tRNAMet | - |
Escherichia coli | |
0.0133 | - |
10-formyltetrahydrofolate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ | Escherichia coli | |
K+ | can partially replace Mg2+ | Escherichia coli | |
Mg2+ | requirement | Escherichia coli | |
Mg2+ | optimal concentration: 30 mM | Escherichia coli | |
Na+ | activates, can partially replace Mg2+ | Escherichia coli | |
NH4+ | activates, can partially replace Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
high speed equilibrium sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
over 1500fold purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.14 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 1 month, 30% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli B / ATCC 11303 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAMet | - |
Escherichia coli B / ATCC 11303 | tetrahydrofolate + N-formylmethionyl-tRNAMet | - |
r | |
additional information | no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA | Escherichia coli | ? | - |
? | |
additional information | no formyl group donor: 5,10-methenyltetrahydrofolate | Escherichia coli | ? | - |
? | |
additional information | no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA | Escherichia coli B / ATCC 11303 | ? | - |
? | |
additional information | no formyl group donor: 5,10-methenyltetrahydrofolate | Escherichia coli B / ATCC 11303 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
with abrupt decrease of reaction rate below pH 7.0 | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.4 | about 60% of maximal activity at pH 6.8 and 8.4 | Escherichia coli |
7 | - |
below: rapid decrease in reaction rate | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Escherichia coli | |
additional information | no cofactors: ribonucleotide mono- or triphosphates | Escherichia coli | |
additional information | no cofactors: ATP, GTP | Escherichia coli | |
tetrahydrofolate | - |
Escherichia coli |