Literature summary for 2.1.3.1 extracted from

Shenoy, B.C.; Kumar, G.K.; Samols, D.
Dissection of the biotinyl subunit of transcarboxylase into regions essential for activity and assembly (1993), J. Biol. Chem., 268, 2232-2238.

Search for more literature for 2.1.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
1.3S subunit cloned and expressed in Escherichia coli	Propionibacterium freudenreichii subsp. shermanii

Organism

Organism	UniProt	Comment	Textmining
Propionibacterium freudenreichii subsp. shermanii	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	a series of truncated forms of the 1.3S subunit is prepared and the effect on the first and the second partial reaction and the overall enzyme reaction measured, result: the sequence 59-78 of 1.3S subunit is necessary for the carboxylation of biotin of the 1.3 S subunit, the inability of the truncated peptides to form enzyme complexes explains the activity loss in the overall reaction	Propionibacterium freudenreichii subsp. shermanii

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme and mutant 1.3S subunit	Propionibacterium freudenreichii subsp. shermanii

Reaction

Reaction	Comment	Organism	Reaction ID
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate	mechanism	Propionibacterium freudenreichii subsp. shermanii	a
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate	partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit	Propionibacterium freudenreichii subsp. shermanii	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
propionyl-CoA + oxaloacetate	two partial reactions	Propionibacterium freudenreichii subsp. shermanii	(S)-methylmalonyl-CoA + pyruvate	-	r

Subunits

Subunits	Comment	Organism
More	5S subunit is a dimer of identical monomers, contains Co2+ and Zn2+ and assembles with two 1.3 subunits to form a stable complex termed the 6S subunit	Propionibacterium freudenreichii subsp. shermanii

Cofactor

Cofactor	Comment	Organism	Structure
biotin	requirement	Propionibacterium freudenreichii subsp. shermanii

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Release 2023.1 (January 2023)