Cloned (Comment) | Organism |
---|---|
expression of catalytic subunit in Escherichia coli | Pyrococcus abyssi |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion, 0.002 ml of enzyme solution, 7 mg/ml, in 20 mM Tris-HCl, pH 8.2, 2 mM 2-mercaptoethanol, 300 mM NaCl, is mixed with 0.002 ml reservoir solution consisting of 1.4 M citrate, pH 6.8, X-ray structure to 1.8 A resolution | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(Phosphonoacetyl)-L-aspartate | 0.002 mM, 50% inhibition of catalytic subunit | Pyrococcus abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
Carbamoyl phosphate | recombinant catalytic subunit, at 37°C | Pyrococcus abyssi | |
12.3 | - |
L-aspartate | recombinant catalytic subunit, at 30°C | Pyrococcus abyssi | |
14.1 | - |
L-aspartate | recombinant catalytic subunit, at 45°C | Pyrococcus abyssi | |
15.5 | - |
L-aspartate | recombinant catalytic subunit, at 37°C | Pyrococcus abyssi | |
19.7 | - |
L-aspartate | recombinant catalytic subunit, at 55°C | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoylphosphate + L-aspartate | Pyrococcus abyssi | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | P77918 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Pyrococcus abyssi | phosphate + N-carbamoyl-L-aspartate | - |
? | |
carbamoylphosphate + L-aspartate | - |
Pyrococcus abyssi | phosphate + N-carbamoyl-L-aspartate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
catalytic subunit, half-life: 80 min, half-life of holenzyme: 240 min | Pyrococcus abyssi |
98 | - |
half-life of the holoenzyme is 2.5 times higher than that of the catalytic subunit | Pyrococcus abyssi |