Crystallization (Comment) | Organism |
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use of Methyl-TROSY spectra for assignment for approximately 60% of the observed methyl groups in TROSY maps of ATCase by the divide and conquer method. The combination of all approaches leads to assignments for 86% of the methyl groups, providing a large number of probes of structure and dynamics. The derived assignments are used to interpret chemical shift changes of ATCase upon titration with the nucleotide ATP. Large shift changes in the N-terminal tails of the regulatory chain provide the first evidence for structural perturbations in a region playing a critical role on the effect of nucleotide binding on distal catalytic sites of the allosteric enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
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- |