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Literature summary for 2.1.3.2 extracted from

  • Lei, Z.; Wang, N.; Tan, H.; Zheng, J.; Jia, Z.
    Conformational plasticity of the active site entrance in E. coli aspartate transcarbamoylase and its implication in feedback regulation (2020), Int. J. Mol. Sci., 21, 320 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M MES, pH 6.0, 10% (v/v) glycerol, and 10% (w/v) PEG 8000 Escherichia coli

Protein Variants

Protein Variants Comment Organism
H107A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
H107A/Y197A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
R167A inactive Escherichia coli
Y197A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
Y197F the mutant shows reduced activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.84
-
 Carbamoyl phosphate wild type enzyme, at pH 8.3 and 25°C Escherichia coli
19.22
-
 Carbamoyl phosphate mutant enzyme Y197A, at pH 8.3 and 25°C Escherichia coli
32.98
-
 Carbamoyl phosphate mutant enzyme Y197F, at pH 8.3 and 25°C Escherichia coli
38.76
-
 Carbamoyl phosphate mutant enzyme H170A, at pH 8.3 and 25°C Escherichia coli
66.37
-
 Carbamoyl phosphate mutant enzyme H170A/Y197A, at pH 8.3 and 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + L-aspartate Escherichia coli
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A786
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
dodecamer composed of two catalytic trimers and three regulatory dimers Escherichia coli

Synonyms

Synonyms Comment Organism
aspartate transcarbamoylase
-
 Escherichia coli
ATCase
-
 Escherichia coli