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Literature summary for 2.1.3.3 extracted from
- Enzymic conversion of agmatine to putrescine in Lathyrus sativus seedlings. Purification and properties of a multifunctional enzyme (putrescine synthase) (1981), J. Biol. Chem., 256, 9532-9541.
General Stability
| General Stability | Organism |
|---|---|
| prolonged dialysis and freeze-thawing causes loss of activity | Lathyrus sativus |
| purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+ | Lathyrus sativus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Lathyrus sativus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
1 * 55000, multifunctional enzyme, SDS-PAGE | Lathyrus sativus |
| 56000 | - |
multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration | Lathyrus sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lathyrus sativus | - |
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column | Lathyrus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Lathyrus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.47 | - |
multifunctional enzyme | Lathyrus sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 48 h, complete loss of activity | Lathyrus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | enzyme also catalyzes the reactions of EC 2.1.3.6, EC 2.7.2.2 and EC 3.5.3.12, thus acting as putrescine synthase converting agmatine and ornithine into putrescine and citrulline | Lathyrus sativus | phosphate + L-citrulline | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, multifunctional enzyme, SDS-PAGE | Lathyrus sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Lathyrus sativus |
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