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Literature summary for 2.1.3.3 extracted from

  • Legrain, C.; Stalon, V.
    Ornithine carbamoyltransferase from Escherichia coli W. Purification, structure and steady-state kinetic analysis (1976), Eur. J. Biochem., 63, 289-301.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,4-diaminobutyrate
-
Escherichia coli
arsenate competitive vs. carbamoylphosphate, noncompetitive vs. ornithine Escherichia coli
glycine
-
Escherichia coli
L-2,4-diaminobutyric acid
-
Escherichia coli
L-alanine
-
Escherichia coli
L-asparagine weak Escherichia coli
L-isoleucine
-
Escherichia coli
L-leucine
-
Escherichia coli
L-lysine weak Escherichia coli
L-norvaline
-
Escherichia coli
L-serine weak Escherichia coli
L-valine
-
Escherichia coli
methionine L-isomer Escherichia coli
ornithine at high concentrations; noncompetitive substrate inhibition vs. carbamoylphosphate Escherichia coli
phosphate competitive vs. carbamoylphosphate, noncompetitive vs. ornithine Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
3 * 35000, SDS-PAGE Escherichia coli
105000
-
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + L-ornithine Escherichia coli enzyme belongs to the arginine biosynthesis pathway phosphate + L-citrulline
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat, ammonium sulfate, DEAE-Sephadex, Sephadex G-200, aminohexyl-Sepharose Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2833
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
Escherichia coli phosphate + L-citrulline
-
r
carbamoyl phosphate + L-ornithine enzyme belongs to the arginine biosynthesis pathway Escherichia coli phosphate + L-citrulline
-
r

Subunits

Subunits Comment Organism
trimer 3 * 35000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
half-life: 5 min, 8 min with 500 mM KCl, 10 min with 10 mM citrulline, 20 min with 100 mM citrulline, 43 min with 20 mM phosphate, 48 min with 100 mM ornithine, 108 min with 100 mM phosphate, 120 min with 10 mM phosphate and 10 mM ornithine, 122 min with 10 mM phosphate and 100 mM citrulline, more than 200 min with 100 mM phosphate and 100 mM citrulline or with 10 mM phosphate and 100 mM ornithine Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
L-norvaline
-
Escherichia coli
5.5
-
L-leucine
-
Escherichia coli
7
-
2,4-diaminobutyrate
-
Escherichia coli
13
-
L-alanine
-
Escherichia coli
13.5
-
L-valine
-
Escherichia coli
14
-
L-isoleucine
-
Escherichia coli
15
-
glycine
-
Escherichia coli
40
-
L-methionine
-
Escherichia coli
63.5
-
L-serine
-
Escherichia coli
110
-
L-asparagine
-
Escherichia coli