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Literature summary for 2.1.3.6 extracted from
- Structure and properties of the putrescine carbamoyltransferase of Streptococcus faecalis (1979), Eur. J. Biochem., 101, 143-12.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Enterococcus faecalis |  |
| spermidine | - |
Enterococcus faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
putrescine | - |
Enterococcus faecalis | |
| 0.35 | - |
spermine | - |
Enterococcus faecalis | |
| 2.9 | - |
1,3-diaminopropane | - |
Enterococcus faecalis | |
| 6.6 | - |
1,6-diaminohexane | - |
Enterococcus faecalis | |
| 7.7 | - |
cadaverine | - |
Enterococcus faecalis | |
| 13 | - |
ornithine | - |
Enterococcus faecalis | |
| 19.7 | - |
spermidine | - |
Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
3 * 40000, treatment with glutaraldehyde, SDS-PAGE | Enterococcus faecalis |
| 140000 | - |
gel filtration | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 508.3 | - |
- |
Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + 1,3-diaminopropane | - |
Enterococcus faecalis | phosphate + N1-carbamoyl-1,3-diaminopropane | - |
? | |
| carbamoyl phosphate + 1,6-diaminohexane | - |
Enterococcus faecalis | phosphate + N1-carbamoyl-1,6-diaminohexane | - |
? | |
| carbamoyl phosphate + cadaverine | - |
Enterococcus faecalis | phosphate + N-carbamoylcadaverine | - |
? | |
| carbamoyl phosphate + spermidine | - |
Enterococcus faecalis | phosphate + N-carbamoylspermidine | - |
? | |
| carbamoyl phosphate + spermine | - |
Enterococcus faecalis | phosphate + N-carbamoylspermine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 40000, treatment with glutaraldehyde, SDS-PAGE | Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
carbamoylphosphate, diphosphate, ornithine, 2,4-diaminobutyrate and norvaline protect against denaturation | Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Enterococcus faecalis |
| 9 | - |
- |
Enterococcus faecalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
spermidine | vs. putrescine | Enterococcus faecalis | |
| 4.5 | - |
spermidine | vs. carbamoylphosphate | Enterococcus faecalis | |
| 6 | - |
phosphate | vs. carbamoylphosphate | Enterococcus faecalis | |
| 8.5 | - |
phosphate | vs. putrescine | Enterococcus faecalis | |
| 13.5 | - |
phosphate | vs. ornithine | Enterococcus faecalis | |
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