Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Pseudomonas savastanoi pv. phaseolicola |
Protein Variants | Comment | Organism |
---|---|---|
M243P/H244N | there is no detectable difference about catalytic efficiency between the mutant and the wild type enzyme | Pseudomonas savastanoi pv. phaseolicola |
M243P/H244N | the mutation has no effect on substrate specificity or catalytic efficiency compared to the wild type enzyme | Pseudomonas savastanoi pv. phaseolicola |
M246S | the mutation abolishes the enzyme activity | Pseudomonas savastanoi pv. phaseolicola |
M246S | the mutation abolishes amidinotransferase activity | Pseudomonas savastanoi pv. phaseolicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + L-lysine | Pseudomonas savastanoi pv. phaseolicola | - |
L-ornithine + L-homoarginine | - |
r | |
L-arginine + L-lysine | Pseudomonas savastanoi pv. phaseolicola 1448A | - |
L-ornithine + L-homoarginine | - |
r | |
L-ornithine + L-homoarginine | Pseudomonas savastanoi pv. phaseolicola | - |
L-arginine + L-lysine | - |
r | |
L-ornithine + L-homoarginine | Pseudomonas savastanoi pv. phaseolicola 1448A | - |
L-arginine + L-lysine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas savastanoi pv. phaseolicola | - |
- |
- |
Pseudomonas savastanoi pv. phaseolicola 1448A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas savastanoi pv. phaseolicola |
ammonium sulfate precipitation, Ni-NTA column chromatography and Superdex S200 gel filtration | Pseudomonas savastanoi pv. phaseolicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + L-lysine | - |
Pseudomonas savastanoi pv. phaseolicola | L-ornithine + L-homoarginine | - |
r | |
L-arginine + L-lysine | - |
Pseudomonas savastanoi pv. phaseolicola 1448A | L-ornithine + L-homoarginine | - |
r | |
L-ornithine + L-canavanine | - |
Pseudomonas savastanoi pv. phaseolicola | L-arginine + ? | - |
? | |
L-ornithine + L-canavanine | - |
Pseudomonas savastanoi pv. phaseolicola 1448A | L-arginine + ? | - |
? | |
L-ornithine + L-canavanine | - |
Pseudomonas savastanoi pv. phaseolicola | L-arginine + O-aminohomoserine | - |
r | |
L-ornithine + L-homoarginine | - |
Pseudomonas savastanoi pv. phaseolicola | L-arginine + L-lysine | - |
r | |
L-ornithine + L-homoarginine | - |
Pseudomonas savastanoi pv. phaseolicola 1448A | L-arginine + L-lysine | - |
r | |
additional information | no activity with glycine, 6-aminocaproic acid, norleucine and L-2,3-diaminopropionic acid as amidine acceptor or guanidinoacetic acid as amidine donor | Pseudomonas savastanoi pv. phaseolicola | ? | - |
? | |
additional information | no activity with guanidinoacetic acid as amidine donor. No activity with norleucine, 6-aminocaproic acid, glycine and L-Dap as amidine acceptor | Pseudomonas savastanoi pv. phaseolicola | ? | - |
- |
|
additional information | no activity with glycine, 6-aminocaproic acid, norleucine and L-2,3-diaminopropionic acid as amidine acceptor or guanidinoacetic acid as amidine donor | Pseudomonas savastanoi pv. phaseolicola 1448A | ? | - |
? | |
additional information | no activity with guanidinoacetic acid as amidine donor. No activity with norleucine, 6-aminocaproic acid, glycine and L-Dap as amidine acceptor | Pseudomonas savastanoi pv. phaseolicola 1448A | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, His6-tagged enzyme, SDS-PAGE | Pseudomonas savastanoi pv. phaseolicola |
? | x * 40000, His-tagged enzyme, SDS-PAGE | Pseudomonas savastanoi pv. phaseolicola |
Synonyms | Comment | Organism |
---|---|---|
amidinotransferase | - |
Pseudomonas savastanoi pv. phaseolicola |
amtA | - |
Pseudomonas savastanoi pv. phaseolicola |