Crystallization (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Oxythiamine diphosphate | - |
Saccharomyces cerevisiae | |
phosphate | - |
Saccharomyces cerevisiae | |
sulfate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-xylulose 5-phosphate | - |
Saccharomyces cerevisiae | |
0.4 | - |
D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | |
1.8 | - |
fructose 6-phosphate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Sus scrofa | 5829 | - |
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | requirement | Sus scrofa | |
Mg2+ | requirement | Saccharomyces cerevisiae | |
Mg2+ | no exogenous Mg2+-addition necessary | Rattus norvegicus | |
Mg2+ | no exogenous Mg2+-addition necessary | Sus scrofa | |
Mg2+ | no exogenous Mg2+-addition necessary | Saccharomyces cerevisiae | |
Mg2+ | indispensable for thiamine diphosphate/enzyme binding | Rattus norvegicus | |
Mg2+ | indispensable for thiamine diphosphate/enzyme binding | Sus scrofa | |
Mg2+ | indispensable for thiamine diphosphate/enzyme binding | Saccharomyces cerevisiae | |
additional information | - |
Rattus norvegicus | |
additional information | - |
Saccharomyces cerevisiae | |
additional information | no addition of exogenous metal ions required | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
sedimentation equilibrium method | Rattus norvegicus |
138000 | - |
meniscus depletion method | Sus scrofa |
158000 | 159000 | sedimentation equilibrium data | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | Rattus norvegicus | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | Sus scrofa | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | Saccharomyces cerevisiae | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
baker's yeast | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
- |
Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
- |
Sus scrofa |
1.5 | - |
- |
Rattus norvegicus |
20 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, several days | Sus scrofa |
4°C, several weeks in 8 M glycylglycine buffer, pH 7.4 | Saccharomyces cerevisiae |
frozen, at least a week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | L-erythrulose | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | L-erythrulose | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | L-erythrulose | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | glycolaldehyde | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | glycolaldehyde | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | glycolaldehyde | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | and acceptor substrates may be: D-glyceraldehyde 3-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | and acceptor substrates may be: D-glyceraldehyde 3-phosphate | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | and acceptor substrates may be: D-glyceraldehyde 3-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-ribose 5-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-ribose 5-phosphate | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-ribose 5-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Sus scrofa | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
hydroxypyruvate + D-glyceraldehyde 3-phosphate | - |
Rattus norvegicus | CO2 + ribulose 5-phosphate | - |
ir | |
hydroxypyruvate + D-glyceraldehyde 3-phosphate | - |
Sus scrofa | CO2 + ribulose 5-phosphate | - |
ir | |
hydroxypyruvate + D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | CO2 + ribulose 5-phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | at sufficiently low concentration the apo-, not the holoenzyme dissociates reversibly into 2 subunits of equal molecular weight, individual subunits are equally catalytically active | Saccharomyces cerevisiae |
tetramer | alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thiamine diphosphate enhances thermal stability | Sus scrofa |
20 | - |
at pH 6.5-8.7, 20 min stable | Sus scrofa |
40 | - |
pH 8.0, activity hardly changes for 60 min | Sus scrofa |
50 | - |
t1/2: 10 min | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
pI: 7.6-7.8 | Sus scrofa |
7.6 | - |
- |
Rattus norvegicus |
7.6 | - |
- |
Sus scrofa |
7.6 | - |
- |
Saccharomyces cerevisiae |
7.8 | 8.2 | - |
Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
thiamine diphosphate dissociates from holoenzyme | Sus scrofa |
6.5 | 8.7 | 20°C, 20 min stable | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | with different affinities for the cofactor | Rattus norvegicus | |
thiamine diphosphate | with different affinities for the cofactor | Sus scrofa | |
thiamine diphosphate | with different affinities for the cofactor | Saccharomyces cerevisiae | |
thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Rattus norvegicus | |
thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Sus scrofa | |
thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Saccharomyces cerevisiae |