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Literature summary for 2.2.1.1 extracted from

  • Kochetov, G.A.
    Transketolase from yeast, rat liver, and pig liver (1982), Methods Enzymol., 90, 209-223.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Oxythiamine diphosphate
-
Saccharomyces cerevisiae
phosphate
-
Saccharomyces cerevisiae
sulfate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
D-xylulose 5-phosphate
-
Saccharomyces cerevisiae
0.4
-
D-ribose 5-phosphate
-
Saccharomyces cerevisiae
1.8
-
fructose 6-phosphate
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Sus scrofa 5829
-
cytosol
-
Saccharomyces cerevisiae 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Rattus norvegicus
Mg2+ requirement Sus scrofa
Mg2+ requirement Saccharomyces cerevisiae
Mg2+ no exogenous Mg2+-addition necessary Rattus norvegicus
Mg2+ no exogenous Mg2+-addition necessary Sus scrofa
Mg2+ no exogenous Mg2+-addition necessary Saccharomyces cerevisiae
Mg2+ indispensable for thiamine diphosphate/enzyme binding Rattus norvegicus
Mg2+ indispensable for thiamine diphosphate/enzyme binding Sus scrofa
Mg2+ indispensable for thiamine diphosphate/enzyme binding Saccharomyces cerevisiae
additional information
-
Rattus norvegicus
additional information
-
Saccharomyces cerevisiae
additional information no addition of exogenous metal ions required Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
sedimentation equilibrium method Rattus norvegicus
138000
-
meniscus depletion method Sus scrofa
158000 159000 sedimentation equilibrium data Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate Rattus norvegicus
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate Sus scrofa
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate Saccharomyces cerevisiae
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
baker's yeast
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Sus scrofa
-
Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
-
Sus scrofa
1.5
-
-
Rattus norvegicus
20
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, several days Sus scrofa
4°C, several weeks in 8 M glycylglycine buffer, pH 7.4 Saccharomyces cerevisiae
frozen, at least a week Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
Rattus norvegicus CO2 + ribulose 5-phosphate
-
ir
hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
Sus scrofa CO2 + ribulose 5-phosphate
-
ir
hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae CO2 + ribulose 5-phosphate
-
ir

Subunits

Subunits Comment Organism
More at sufficiently low concentration the apo-, not the holoenzyme dissociates reversibly into 2 subunits of equal molecular weight, individual subunits are equally catalytically active Saccharomyces cerevisiae
tetramer alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thiamine diphosphate enhances thermal stability Sus scrofa
20
-
at pH 6.5-8.7, 20 min stable Sus scrofa
40
-
pH 8.0, activity hardly changes for 60 min Sus scrofa
50
-
t1/2: 10 min Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
Rattus norvegicus
additional information
-
-
Saccharomyces cerevisiae
additional information
-
pI: 7.6-7.8 Sus scrofa
7.6
-
-
Rattus norvegicus
7.6
-
-
Sus scrofa
7.6
-
-
Saccharomyces cerevisiae
7.8 8.2
-
Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
thiamine diphosphate dissociates from holoenzyme Sus scrofa
6.5 8.7 20°C, 20 min stable Sus scrofa

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate with different affinities for the cofactor Rattus norvegicus
thiamine diphosphate with different affinities for the cofactor Sus scrofa
thiamine diphosphate with different affinities for the cofactor Saccharomyces cerevisiae
thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Rattus norvegicus
thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Sus scrofa
thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Saccharomyces cerevisiae