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Literature summary for 2.2.1.1 extracted from

  • Zhou, C.; Saravanan, T.; Lorilliere, M.; Wei, D.; Charmantray, F.; Hecquet, L.; Fessner, W.D.; Yi, D.
    Second-generation engineering of a thermostable transketolase (TKGst) for aliphatic aldehyde acceptors with either improved or reversed stereoselectivity (2017), ChemBioChem, 18, 455-459 .
    View publication on PubMed
Search for more literature for 2.2.1.1

Protein Variants

Protein Variants Comment Organism
F435L/D470E the mutant shows a large activity improvement (4.9fold) compared to the wild type enzyme Geobacillus stearothermophilus
F435L/D470E the mutant shows a roughly 4.9fold activity improvement compared to the wild type enzyme Geobacillus stearothermophilus
L191I/D470L the mutant has an activity improvement of roughly 7.4fold compared to the wild type enzyme Geobacillus stearothermophilus
L191V/D470 the mutant exhibits high 3R stereoselectivity as compared to the wild type enzyme Geobacillus stearothermophilus
L191V/D470I the mutant exhibits 74% 3R stereoselectivity and 5.8fold activity improvement as compared to the wild type enzyme Geobacillus stearothermophilus
L191V/D470R the mutant exhibits 84% 3R stereoselectivity as compared to the wild type enzyme Geobacillus stearothermophilus
L382F/D470 the mutant has specific activity towards propanal 11 times higher than that of the wild type enzyme, with high yield of the 3S product Geobacillus stearothermophilus
L382I/D470L the mutant shows inversed stereoselectivity with low yield of the 3R product Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Geobacillus stearothermophilus
-
 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 propanal + 2 lithium beta-hydroxypyruvate
-
 Geobacillus stearothermophilus (3S)-1,3-dihydroxypentan-2-one + (3R)-1,3-dihydroxypentan-2-one + 2 CO2 + 2 Li+
-
 ir
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 Geobacillus stearothermophilus D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 r

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Geobacillus stearothermophilus