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Literature summary for 2.2.1.2 extracted from
- Novel mode of inhibition by D-tagatose 6-phosphate through a Heyns rearrangement in the active site of transaldolase B variants (2016), Acta Crystallogr. Sect. D, 72, 467-476 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) Star pLysS cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant enzyme F178Y with bound D-tagatose 6-phosphate , hanging drop vapor diffusion method, using 0.15 M Li2SO4, 18% (w/v) PEG 3350. Mutant enzyme E96Q with bound D-fructose 6-phosphate, hanging drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 18% (w/v) PEG3350 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E96Q | the mutant enzyme with reduced transaldolase activity is irreversibly inhibited by D-tagatose 6-phosphate | Escherichia coli |
| E96Q/F178Y | the mutant enzyme is irreversibly inhibited by D-tagatose 6-phosphate | Escherichia coli |
| F178Y | the mutant enzyme is inhibited by D-tagatose 6-phosphate | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the wild type enzyme is not inhibited by D-tagatose 6-phosphate | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythrose 4-phosphate + D-fructose 6-phosphate | Escherichia coli | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |  |
| sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | Escherichia coli | - |
D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A870 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
Escherichia coli | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TALB | - |
Escherichia coli |
| transaldolase B | - |
Escherichia coli |
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