Crystallization (Comment) | Organism |
---|---|
mutant enzyme E60Q/F132Y, hanging drop vapor diffusion method, using 200 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
E60Q/F132Y | the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity | Thermoplasma acidophilum |
F132Y | the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
D-fructose 6-phosphate | wild type enzyme, at pH 7.5 and 30°C | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythrose 4-phosphate + D-fructose 6-phosphate | Thermoplasma acidophilum | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | Thermoplasma acidophilum | - |
D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HKI3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
Thermoplasma acidophilum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
additional information | the enzyme exhibits a very small yet detectable D-fructose 6-phosphate aldolase activity | Thermoplasma acidophilum | ? | - |
- |
|
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
Thermoplasma acidophilum | D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TAL | - |
Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
D-fructose 6-phosphate | mutant enzyme F132Y, at pH 7.5 and 30°C | Thermoplasma acidophilum | |
0.62 | - |
D-fructose 6-phosphate | mutant enzyme E60Q/F132Y, at pH 7.5 and 30°C | Thermoplasma acidophilum | |
13.86 | - |
D-fructose 6-phosphate | wild type enzyme, at pH 7.5 and 30°C | Thermoplasma acidophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.0261 | - |
D-fructose 6-phosphate | wild type enzyme, at pH 7.5 and 30°C | Thermoplasma acidophilum |