Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Avidin | - |
Sus scrofa | |
mercaptoethanol | stimulates | Sus scrofa | |
sucrose | stimulates | Sus scrofa |
General Stability | Organism |
---|---|
mercaptoethanol stabilises | Sus scrofa |
Mg2+ stabilises | Sus scrofa |
sucrose stabilises | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 10 mM | Sus scrofa | |
N-ethylmaleimide | 74% inhibition at 0.01 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
glyoxylate | - |
Sus scrofa | |
16 | - |
2-oxoglutarate | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | stimulates | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Oxidation Stability | Organism |
---|---|
access of air destabilises | Sus scrofa |
Purification (Comment) | Organism |
---|---|
partial | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2 | various alternative mechanisms proposed | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.0, 5 days | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + glyoxylate | - |
Sus scrofa | 2-hydroxy-3-oxohexandioate + CO2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Sus scrofa |