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Literature summary for 2.2.1.5 extracted from

  • Steward, P.R.; Quale, J.R.
    The synergistic decarboxylation of glyoxylate and 2-oxoglutarate by an enzyme system from pig-liver mitochondria (1967), Biochem. J., 102, 885-897.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Avidin
-
Sus scrofa
mercaptoethanol stimulates Sus scrofa
sucrose stimulates Sus scrofa

General Stability

General Stability Organism
mercaptoethanol stabilises Sus scrofa
Mg2+ stabilises Sus scrofa
sucrose stabilises Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 10 mM Sus scrofa
N-ethylmaleimide 74% inhibition at 0.01 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
glyoxylate
-
Sus scrofa
16
-
2-oxoglutarate
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- stimulates Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Oxidation Stability

Oxidation Stability Organism
access of air destabilises Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2 various alternative mechanisms proposed Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.0, 5 days Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + glyoxylate
-
Sus scrofa 2-hydroxy-3-oxohexandioate + CO2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Sus scrofa