Protein Variants | Comment | Organism |
---|---|---|
H351F | 5fold increase in Km-value, weak resistance to Londax and Cadre, difference in secondary structure compared to wild-type | Nicotiana tabacum |
H351M | 18fold increase in Km-value, strong resistance to Londax and Cadre, difference in secondary structure compared to wild-type | Nicotiana tabacum |
H351Q | 60fold increase in Km-value, strong resistance to Londax and Cadre, difference in secondary structure compared to wild-type | Nicotiana tabacum |
H392M | no significant effects | Nicotiana tabacum |
H487F | no enzymic activity, no affinity for FAD | Nicotiana tabacum |
H487L | no enzymic activity, no affinity for FAD | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | i.e. Cadre | Nicotiana tabacum | |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | i.e. TP | Nicotiana tabacum | |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | ratio 1:1, i.e. Londax | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.71 | - |
pyruvate | mutant H392M, 37°C, pH 7.5 | Nicotiana tabacum | |
16.09 | - |
pyruvate | wild-type, 37°C, pH 7.5 | Nicotiana tabacum | |
76.63 | - |
pyruvate | mutantH351F, 37°C, pH 7.5 | Nicotiana tabacum | |
287.8 | - |
pyruvate | mutant H351M, 37°C, pH 7.5 | Nicotiana tabacum | |
959.5 | - |
pyruvate | mutant H351Q, 37°C, pH 7.5 | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
expression in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
mutant H351Q, 37°C, pH 7.5 | Nicotiana tabacum |
0.04 | - |
mutant H351M, 37°C, pH 7.5 | Nicotiana tabacum |
1.58 | - |
mutantH351F, 37°C, pH 7.5 | Nicotiana tabacum |
3.23 | - |
mutant H392M, 37°C, pH 7.5 | Nicotiana tabacum |
5.78 | - |
wild-type, 37°C, pH 7.5 | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000045 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutantH351F, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00000311 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | wild-type, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00000365 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant H392M, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00000368 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant H351M, 37°C, pH 7.5 | Nicotiana tabacum | |
0.0000093 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | mutant H392M, 37°C, pH 7.5 | Nicotiana tabacum | |
0.0000109 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | wild-type, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00002304 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | mutantH351F, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00004857 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant H351Q, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00135 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | wild-type, 37°C, pH 7.5 | Nicotiana tabacum | |
0.00205 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant H392M, 37°C, pH 7.5 | Nicotiana tabacum | |
0.01735 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutantH351F, 37°C, pH 7.5 | Nicotiana tabacum |