Protein Variants | Comment | Organism |
---|---|---|
D374A | substrate inhibition at high concentrations, strong resistance to Londax and Cadre, 10fold increase in activation efficiency for thiamine diphosphate | Nicotiana tabacum |
D374A/D375A | strong resistance to Londax and to C, about 2fold increase in affinity for FAD, decrease in activation efficiency for thiamine diphosphate | Nicotiana tabacum |
D374E | greatly reduced activity, strong resistance to Londax, 8fold increase in affinity for FAD, decrease in activation efficiency for thiamine diphosphate | Nicotiana tabacum |
D374E/D375E | strong resistance to Londax and to C | Nicotiana tabacum |
D375A | about 10fold increase in Km-value, strong resistance to Londax | Nicotiana tabacum |
D375E | about 3fold reduction in Km-value, strong resistance to Londax, about 3fold increase in activation efficiency of FAD | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | i.e. Cadre | Nicotiana tabacum | |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | - |
Nicotiana tabacum | |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | ratio 1:1, i.e. Londax | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
pyruvate | mutant D375E, pH 7.5, 37°C | Nicotiana tabacum | |
5.7 | - |
pyruvate | mutant D374A, pH 7.5, 37°C | Nicotiana tabacum | |
11.7 | - |
pyruvate | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
55.8 | - |
pyruvate | mutant D374A/D375A, pH 7.5, 37°C | Nicotiana tabacum | |
109.3 | - |
pyruvate | mutant D375A, pH 7.5, 37°C | Nicotiana tabacum | |
266.7 | - |
pyruvate | mutant D374E/D375E, pH 7.5, 37°C | Nicotiana tabacum | |
557.6 | - |
pyruvate | mutant D374E, pH 7.5, 37°C | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
mutant D374A/D375A, pH 7.5, 37°C | Nicotiana tabacum |
0.64 | - |
mutant D374E/D375E, pH 7.5, 37°C | Nicotiana tabacum |
0.65 | - |
mutant D375A, pH 7.5, 37°C | Nicotiana tabacum |
0.77 | - |
mutant D374E, pH 7.5, 37°C | Nicotiana tabacum |
0.91 | - |
mutant D374A, pH 7.5, 37°C | Nicotiana tabacum |
1.53 | - |
mutant D375E, pH 7.5, 37°C | Nicotiana tabacum |
1.55 | - |
wild-type, pH 7.5, 37°C | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum | |
thiamine diphosphate | - |
Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.000108 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | mutant D374E, pH 7.5, 37°C | Nicotiana tabacum | |
0.0008 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D375A, pH 7.5, 37°C | Nicotiana tabacum | |
0.00086 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | mutant D374A, pH 7.5, 37°C | Nicotiana tabacum | |
0.0041 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.0133 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D374A/D375A, pH 7.5, 37°C | Nicotiana tabacum | |
0.0155 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D374E, pH 7.5, 37°C | Nicotiana tabacum | |
0.0293 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.0296 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D374E/D375E, pH 7.5, 37°C | Nicotiana tabacum | |
0.036 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant D374E, pH 7.5, 37°C | Nicotiana tabacum | |
0.0389 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D375E, pH 7.5, 37°C | Nicotiana tabacum | |
0.0741 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant D374A, pH 7.5, 37°C | Nicotiana tabacum | |
0.181 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant D374A, pH 7.5, 37°C | Nicotiana tabacum | |
0.198 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant D375A, pH 7.5, 37°C | Nicotiana tabacum | |
0.309 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant D374A/D375A, pH 7.5, 37°C | Nicotiana tabacum | |
0.79 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant D374E/D375E, pH 7.5, 37°C | Nicotiana tabacum |