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Literature summary for 2.2.1.6 extracted from

  • Tittmann, K.; Vyazmensky, M.; Hubner, G.; Barak, Z.; Chipman, D.M.
    The carboligation reaction of acetohydroxyacid synthase II: steady-state intermediate distributions in wild type and mutants by NMR (2005), Proc. Natl. Acad. Sci. USA, 102, 553-558.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.2.1.6

Protein Variants

Protein Variants Comment Organism
M250A large decrease in activity, increase in Km-value Escherichia coli
R276K large decrease in activity, increase in Km-value Escherichia coli
W464L decrease in activity, increase in Km-value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
 pyruvate wild-type, pH 7.6, 37°C Escherichia coli
6
-
 pyruvate wild-type with His-tag, pH 7.6, 37°C Escherichia coli
11.2
-
 pyruvate mutant W464L with His-tag, pH 7.6, 37°C Escherichia coli
36
-
 pyruvate mutant M250A with His-tag, pH 7.6, 37°C Escherichia coli
38
-
 pyruvate mutant R276K with His-tag, pH 7.6, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 pyruvate = 2-acetolactate + CO2 specificity of carboligation and product liberation may be cumulative if the former is not completely committed Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
 mutant M250A with His-tag, pH 7.6, 37°C Escherichia coli
2.8
-
 mutant R276K with His-tag, pH 7.6, 37°C Escherichia coli
11.9
-
 mutant W464L with His-tag, pH 7.6, 37°C Escherichia coli
17
-
 wild-type with His-tag, pH 7.6, 37°C Escherichia coli
20
-
 wild-type, pH 7.6, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Escherichia coli 2-acetolactate + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.03
-
 pyruvate mutant M250A with His-tag, pH 7.6, 37°C Escherichia coli
3.27
-
 pyruvate mutant R276K with His-tag, pH 7.6, 37°C Escherichia coli
13.9
-
 pyruvate mutant W464L with His-tag, pH 7.6, 37°C Escherichia coli
19.8
-
 pyruvate wild-type with His-tag, pH 7.6, 37°C Escherichia coli
23.3
-
 pyruvate wild-type, pH 7.6, 37°C Escherichia coli