Protein Variants | Comment | Organism |
---|---|---|
Q487A | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
Q487A | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
Q487G | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
Q487G | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
Q487I | complete loss of synthase activity | Bacillus subtilis |
Q487I | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
Q487L | complete loss of synthase activity | Bacillus subtilis |
Q487L | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
Q487S | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
Q487S | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
1.6 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
8.7 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
13.6 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | Q04789 | - |
- |
Bacillus subtilis 168 | Q04789 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Bacillus subtilis | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Bacillus subtilis 168 | 2-acetolactate + CO2 | - |
? | |
additional information | enzyme additionallly displays 2-ketoisovalerate decarboxylase activity | Bacillus subtilis | ? | - |
? | |
additional information | acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis | ? | - |
? | |
additional information | acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis 168 | ? | - |
? | |
pyruvate | - |
Bacillus subtilis | (S)-2-acetolactate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlsS | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
11 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
58 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
121 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
6.9 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
8.9 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
10 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis |