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Literature summary for 2.2.1.6 extracted from

  • Choi, K.; Pham, C.N.; Jung, H.; Han, S.; Choi, J.; Kim, J.; Yoon, M.
    Expression of acetohydroxyacid synthase from Bacillus anthracis and its potent inhibitors (2007), Bull. Korean Chem. Soc., 28, 1109-1113.
No PubMed abstract available
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene AHAS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), the enzyme contains additional 34 residues, MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS, at the N-terminus Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
Cadre an imidazolinone herbicide Bacillus anthracis
Londax a sulfonylurea herbicide Bacillus anthracis
additional information AHAS from Bacillus anthracis shows strong resistance to three classes of herbicides, the sulfonylurea Londax, the imidazolinone Cadre, and the triazolopyrimidine TP Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 thiamine diphosphate
-
 Bacillus anthracis
4.8
-
 pyruvate pH 7.4, 37°C, recombinant enzyme Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ K0.5 is 1.160 mM Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, recombinant enzyme, SDS-PAGE Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Bacillus anthracis acetohydroxyacid synthase is the enzyme that catalyses the first step in the common pathway of the biosynthesis of the branched chain amino acids, valine, leucine and isoleucine 2-acetolactate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to 96% purity Bacillus anthracis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
 calculated per subunit, purified recombinant His-tagged enzyme Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Bacillus anthracis 2-acetolactate + CO2
-
 ?
2 pyruvate acetohydroxyacid synthase is the enzyme that catalyses the first step in the common pathway of the biosynthesis of the branched chain amino acids, valine, leucine and isoleucine Bacillus anthracis 2-acetolactate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, recombinant enzyme, SDS-PAGE Bacillus anthracis

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase
-
 Bacillus anthracis
AHAS
-
 Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus anthracis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, temperature profile Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus anthracis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 6.5: about 40% of maximal activity, pH 10.0: about 65% of maximal activity, pH profile Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Bacillus anthracis
thiamine diphosphate
-
 Bacillus anthracis