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Literature summary for 2.2.1.6 extracted from

  • Piao, Z.; Wang, W.; Wei, Y.; Zonta, F.; Wan, C.; Bai, J.; Wu, S.; Wang, X.; Fang, J.
    Characterization of an acetohydroxy acid synthase mutant conferring tolerance to imidazolinone herbicides in rice (Oryza sativa) (2018), Planta, 247, 693-703 .
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Oryza sativa subsp. japonica variety JD164 and Escherichia coli Rosetta cells Oryza sativa Indica Group

Protein Variants

Protein Variants Comment Organism
S627N the mutation confers tolerance against imidazolinone herbicides, including imazethapyr and imazamox. The mutant is tolerant to imidazolinone but the catalytic efficiency of the mutated enzyme decreases in its presence. Moreover, the activity of the mutated enzyme decreases more in the presence of imazethapyr than in the presence of imazamox Oryza sativa Indica Group

Inhibitors

Inhibitors Comment Organism Structure
bispyribac
-
 Oryza sativa Indica Group
chlorsulfuron
-
 Oryza sativa Indica Group
imazamox
-
 Oryza sativa Indica Group
imazethapyr
-
 Oryza sativa Indica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.5
-
 pyruvate wild type enzyme, at pH 7.5 and 20°C Oryza sativa Indica Group
8.8
-
 pyruvate mutant enzyme S627N, at pH 7.5 and 20°C Oryza sativa Indica Group
9
-
 pyruvate mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C Oryza sativa Indica Group
11.4
-
 pyruvate mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C Oryza sativa Indica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Oryza sativa Indica Group
-
 2-acetolactate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Indica Group A5X300
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Oryza sativa Indica Group 2-acetolactate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, mature enzyme, SDS-PAGE Oryza sativa Indica Group

Synonyms

Synonyms Comment Organism
acetohydroxy acid synthase
-
 Oryza sativa Indica Group
acetolactate synthase
-
 Oryza sativa Indica Group
AHAS
-
 Oryza sativa Indica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
 pyruvate mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C Oryza sativa Indica Group
4.1
-
 pyruvate mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C Oryza sativa Indica Group
4.5
-
 pyruvate wild type enzyme, at pH 7.5 and 20°C Oryza sativa Indica Group
4.6
-
 pyruvate mutant enzyme S627N, at pH 7.5 and 20°C Oryza sativa Indica Group
8.8
-
 pyruvate wild type enzyme, at pH 7.5 and 20°C Oryza sativa Indica Group

Expression

Organism Comment Expression
Oryza sativa Indica Group the mutant enzyme S627N mRNA level is increased after imazethapyr or imazamox application up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.32
-
 pyruvate wild type enzyme, at pH 7.5 and 20°C Oryza sativa Indica Group
0.46
-
 pyruvate mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C Oryza sativa Indica Group
0.52
-
 pyruvate mutant enzyme S627N, at pH 7.5 and 20°C Oryza sativa Indica Group
11.4
-
 pyruvate mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C Oryza sativa Indica Group